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High-performance liquid chromatographic determination of dietary fibre in raw and processed carrots
A Redondo1, M J Villanueva, M D Rodríguez
1Departamento de Nutrición y Bromatología II, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Journal of Chromatography. A
|August 19, 1994
Abstract:
An HPLC method was developed to determine dietary fibre in carrots (Daucus carota L.). Primary hydrolysis of dietary fibre residue was performed with 12 M H2SO4 for 2 h at 40 degrees C and secondary hydrolysis with 0.414 M H2SO4 for 3 h at 100 degrees C. For the neutralization step prior to injection, AG4-X4 resin (Bio-Rad) was used. Neutral monosaccharides were separated using an HPX-87P column (Bio-Rad). This method was applied for evaluation of the quantitative variation of dietary fibre content in carrots during autoclaving.