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Related Experiment Videos

Comparison of progenitor cell concentration techniques: continuous flow separation versus density-gradient isolation

J M Davis1, K G Schepers, L L Eby

  • 1Johns Hopkins Oncology Center, Baltimore, MD 21287-8973.

Journal of Hematotherapy
|January 1, 1993
PubMed
Summary

The COBE Spectra method for preparing mononuclear cell (MNC) concentrates is a safe and effective alternative to the COBE 2991 density-gradient technique for bone marrow processing, especially when red blood cells are not a concern.

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Area of Science:

  • Hematology
  • Cellular Therapy
  • Biotechnology

Background:

  • Bone marrow processing is crucial for cell-based therapies.
  • Current methods include density-gradient separation and automated cell concentration.
  • Evaluating new techniques for efficiency and safety is essential.

Purpose of the Study:

  • To compare the efficacy and safety of mononuclear cell (MNC) concentrate preparation using the COBE Spectra system versus the COBE 2991 density-gradient method.
  • To assess progenitor cell recovery and product purity for both techniques.

Main Methods:

  • Bone marrow harvests were processed using two methods: COBE Spectra for MNC concentrate and COBE 2991 for light-density cell fractions.
  • Nucleated cell recovery, progenitor cell recovery (CFU-GM), and CD34+ cell recovery were compared.

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  • Product characteristics such as hematocrit and granulocyte contamination were analyzed.

    • Main Results:

    • Both methods yielded similar nucleated cell recovery (22% vs. 21%).
    • COBE Spectra showed comparable or superior progenitor cell recovery (132% CFU-GM, 101% CD34+) versus COBE 2991 (100% CFU-GM, 104% CD34+).
    • Spectra produced MNCs with less granulocyte contamination and higher MNC percentage, though with a measurable hematocrit (4%) that could interfere with certain downstream processes.

    Conclusions:

    • The COBE Spectra system offers a safe substitute for light-density cell preparations when erythrocytes do not impede processing.
    • Spectra's MNC concentrate is prepared with FDA-approved reagents.
    • The Spectra method demonstrates advantages in MNC recovery and purity over the density-gradient technique.