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Related Experiment Videos

Microinjected profilin affects cytoplasmic streaming in plant cells by rapidly depolymerizing actin microfilaments

C J Staiger1, M Yuan, R Valenta

  • 1Department of Cell Biology, John Innes Centre for Plant Science Research, Norwich, UK.

Current Biology : CB
|March 1, 1994
PubMed
Summary

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Plant profilin, a protein regulating actin dynamics, was injected into plant cells. It rapidly disrupted actin filaments, causing cytoplasmic streaming to cease, revealing its role in plant cell organization.

Area of Science:

  • Plant Cell Biology
  • Cytoskeletal Dynamics
  • Molecular Plant Science

Background:

  • Cytoplasmic streaming in plant cells involves organelles moving along actin-filled strands.
  • Mechanisms regulating streaming and actin network formation are poorly understood, likely involving actin-binding proteins.
  • Profilin, a known actin-binding protein in animals, was identified in plants.

Purpose of the Study:

  • To investigate if plant profilin functions as a monomeric actin-binding protein.
  • To determine profilin's influence on actin dynamics in living plant cells in vivo.

Main Methods:

  • Purification of recombinant birch-pollen profilin using polyproline affinity chromatography.
  • Microinjection of profilin into Tradescantia blossfeldiana stamen hair cells.

Related Experiment Videos

  • Assessment of cellular organization and actin filament depolymerization using fluorescein-labelled-phalloidin.

    • Main Results:

    • Microinjection of profilin caused rapid, irreversible changes: thinning and snapping of cytoplasmic strands.
    • Cytoplasmic streaming ceased within 1-3 minutes post-injection.
    • Phalloidin staining confirmed actin filament depolymerization; DNase I injection yielded similar results, supporting actin sequestration.

    Conclusions:

    • Plant profilin acts as a potent regulator of actin organization in living plant cells.
    • Profilin's rapid effect on cytoplasmic strands and streaming supports a model of dynamic actin equilibrium.
    • Organelle movement in plant cells depends on microfilaments in equilibrium with monomeric actin.