Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Transcriptional activation. How lambda repressor talks to RNA polymerase

A Hochschild1

  • 1Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.

Current Biology : CB
|May 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A regulator that inhibits transcription by targeting an intersubunit interaction of the RNA polymerase holoenzyme.

Proceedings of the National Academy of Sciences of the United States of America·2004
Same author

Bacterial two-hybrid analysis of interactions between region 4 of the sigma(70) subunit of RNA polymerase and the transcriptional regulators Rsd from Escherichia coli and AlgQ from Pseudomonas aeruginosa.

Journal of bacteriology·2001
Same author

A chimeric activator of transcription that uses two DNA-binding domains to make simultaneous contact with pairs of recognition sites.

Molecular microbiology·2001
Same author

Magnitude of the CREB-dependent transcriptional response is determined by the strength of the interaction between the kinase-inducible domain of CREB and the KIX domain of CREB-binding protein.

Molecular and cellular biology·2000
Same author

Mechanism for a transcriptional activator that works at the isomerization step.

Proceedings of the National Academy of Sciences of the United States of America·2000
Same author

Crystal structure of the lambda repressor C-terminal domain provides a model for cooperative operator binding.

Cell·2000

Bacteriophage lambda repressor protein controls gene activity. Changes in the RNA polymerase sigma subunit influence how this repressor activates transcription, revealing key insights into the activation mechanism.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The bacteriophage lambda repressor protein plays a crucial role in regulating gene expression.
  • This repressor can either activate or repress transcription initiation.
  • Understanding the precise mechanisms of transcriptional regulation is fundamental in molecular biology.

Purpose of the Study:

  • To investigate the role of the sigma subunit of RNA polymerase in bacteriophage lambda repressor-mediated transcriptional activation.
  • To elucidate the molecular details of how repressor-stimulated transcription is affected by alterations in the sigma subunit.

Main Methods:

  • Site-directed mutagenesis was used to introduce amino-acid substitutions into the sigma subunit of RNA polymerase.
  • In vitro transcription assays were performed to measure repressor-stimulated transcription levels.

Related Experiment Videos

  • Analysis of repressor-DNA binding and its interaction with the modified RNA polymerase.

    • Main Results:

    • Specific amino-acid substitutions within the sigma subunit significantly altered the efficiency of repressor-stimulated transcription.
    • The data indicate that the sigma subunit directly interacts with the lambda repressor during the activation process.
    • Certain mutations in the sigma subunit enhanced or diminished the repressor's ability to activate transcription.

    Conclusions:

    • The sigma subunit of RNA polymerase is a critical component in the mechanism of bacteriophage lambda repressor-mediated transcriptional activation.
    • Amino-acid substitutions in the sigma subunit provide valuable tools for dissecting the protein-protein interactions involved in transcription regulation.
    • These findings contribute to a deeper understanding of the fundamental processes governing gene expression.