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Antisense oligodeoxynucleotides to c-jun inhibits proliferation of transformed NIH 3T3 cells induced by E5a of HPV-11

S L Chen1, L T Tsao, Y P Tsao

  • 1Department of Microbiology and Immunology National Defense Medical Center, Taipei, Taiwan, ROC.

Cancer Letters
|September 30, 1994
PubMed

Insights

Human papillomavirus type 11 (HPV-11) E5a oncogene drives cell transformation. This study shows that inhibiting c-jun production with antisense oligodeoxynucleotides halts proliferation in E5a-transformed cells.

Area of Science:

  • Oncogenomics
  • Molecular Virology

Background:

  • The E5a gene of Human Papillomavirus type 11 (HPV-11) is a transforming oncogene.
  • Previous research indicated E5a's role in initiating cell transformation, with c-jun potentially involved in maintaining this state.

Purpose of the Study:

  • To investigate the role of c-jun in the maintenance of E5a-mediated cell transformation.
  • To determine the effect of inhibiting c-jun production on the proliferation of E5a-transformed cells.

Main Methods:

  • NIH 3T3 cells transformed by HPV-11 E5a were treated with antisense oligodeoxynucleotides.
  • These oligodeoxynucleotides were designed to be complementary to the translation initiation site of the c-jun gene.
  • Cell proliferation was monitored following the treatment.

Main Results:

  • Antisense c-jun oligodeoxynucleotides successfully repressed the production of c-jun protein.
  • This repression of c-jun led to a significant inhibition of cell proliferation in E5a-transformed NIH 3T3 cells.

Conclusions:

  • c-jun plays a crucial role in maintaining the transformed phenotype induced by HPV-11 E5a.
  • Targeting c-jun production offers a potential therapeutic strategy for HPV-11-associated cancers.

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