Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nucleoprotein localization by bismuth staining

G L Brown, M Locke

    Tissue & Cell
    |January 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Bismuth binds to mouse liver nucleoproteins via amino and phosphate groups. This interaction aids in localizing proteins crucial for gene regulation and chromatin structure.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Success and Survival of Composite Resin Restorations for the Management of Localized Anterior Tooth Wear: A Systematic Review and Meta-Analysis.

    The European journal of prosthodontics and restorative dentistry·2024
    Same author

    Increased urokinase and consumption of α<sub>2</sub> -antiplasmin as an explanation for the loss of benefit of tranexamic acid after treatment delay.

    Journal of thrombosis and haemostasis : JTH·2018
    Same author

    Teaching of implant dentistry in undergraduate dental schools in the UK and Ireland.

    British dental journal·2018
    Same author

    An international collaborative study to calibrate the WHO 2nd International Standard for Ancrod (15/106) and the WHO Reference Reagent for Batroxobin (15/140): communication from the SSC of the ISTH.

    Journal of thrombosis and haemostasis : JTH·2018
    Same author

    Salmonella Enteritidis with double deletion in phoP fliC and a competitive exclusion culture elicit substantial additive protective effects against Salmonella exposure in newly hatched chicks.

    Vaccine·2017
    Same author

    Pyoderma gangrenosum with pathergy: A potentially significant complication following breast reconstruction.

    Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2017
    Same journal

    Single-cell transcriptomic profiling identifies TIMP1 as a diagnostic marker linked to macrophage ferroptosis in ulcerative colitis.

    Tissue & cell·2026
    Same journal

    From lab to life: Advances in tissue engineering and 3D bioprinting for regenerative female reproductive health.

    Tissue & cell·2026
    Same journal

    Morphological and ultrastructural characterization of the male accessory glands in Moechotypa diphysis (Coleoptera: Cerambycidae).

    Tissue & cell·2026
    Same journal

    Annexin A1 regulates intestinal epithelial homeostasis and NLRP3-associated signaling in experimental type 1 diabetes.

    Tissue & cell·2026
    Same journal

    CD38-driven NAD⁺ depletion governs crypt structure and barrier dysfunction in fluorouracil-induced colonic mucositis: From metabolic collapse to architectural remodeling.

    Tissue & cell·2026
    Same journal

    Dual regulatory roles and mechanisms of Gamma delta T cells in organ fibrosis.

    Tissue & cell·2026
    See all related articles

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Biochemistry

    Background:

    • Nucleoproteins are essential components of chromatin, playing critical roles in gene regulation and structural organization.
    • Understanding the precise interactions of proteins within the nucleus is key to deciphering cellular processes.
    • Heavy metal staining offers potential for high-resolution visualization of nuclear components.

    Purpose of the Study:

    • To investigate the binding sites of bismuth on mouse liver nucleoproteins.
    • To determine the specific interactions of bismuth with different nucleoprotein components.
    • To evaluate bismuth staining as a method for localizing gene regulatory proteins.

    Main Methods:

    • Enzyme digestion of isolated mouse liver nuclei.

    Related Experiment Videos

  • Crosslinking of amino groups and alkaline hydrolysis.
  • Salt and acid extraction of nucleoproteins.
  • Bismuth staining and analysis of binding patterns.

    • Main Results:

    • Bismuth binds to nucleoproteins through interactions with both amino and phosphate groups.
    • Bismuth-amino group interactions were observed on ribonucleoprotein particles, histones, and some non-histone proteins.
    • Bismuth-phosphate binding was specific to distinct species of non-histone proteins.

    Conclusions:

    • Histones not tightly bound to DNA are located on interchromatin granules, presumed transcriptionally active regions.
    • Phosphorylated non-histone proteins are also localized at these active chromatin sites.
    • Heavy metal staining, like bismuth, shows promise for high-resolution localization of nucleoproteins involved in gene regulation and chromatin structure.