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Related Experiment Videos

Serologic testing for reactive arthritis

G T Thomson1, M Alfa, K Orr

  • 1St. Boniface Arthritis Research Unit, Medical Microbiology Department, St. Boniface General Hospital, Winnipeg, Manitoba.

Clinical and Investigative Medicine. Medecine Clinique Et Experimentale
|June 1, 1994
PubMed
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Quantitative antibody tests for Salmonella enteritidis lipopolysaccharide (LPS) show promise for diagnosing reactive arthritis (ReA). IgA anti-LPS is highly specific, distinguishing past Salmonella exposure in ReA patients.

Area of Science:

  • Immunology
  • Microbiology
  • Rheumatology

Background:

  • Reactive arthritis (ReA) can follow Salmonella enteritidis infections.
  • Accurate diagnostic markers for post-Salmonella ReA are needed.
  • Salmonella enteritidis lipopolysaccharide (LPS) is a key antigen involved in the immune response.

Purpose of the Study:

  • To evaluate the diagnostic accuracy of quantitative serum antibody responses to Salmonella enteritidis LPS for post-Salmonella ReA.
  • To determine the sensitivity and specificity of IgG and IgA anti-LPS antibodies.

Main Methods:

  • Serum samples were collected from individuals with and without ReA following a Salmonella enteritidis outbreak.
  • Quantitative isotypic antibodies to Salmonella enteritidis LPS were measured using ELISA.

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  • Receiver operator characteristic (ROC) curves were used to assess diagnostic performance.

    • Main Results:

    • Both IgG and IgA anti-LPS antibodies were elevated in ReA patients compared to controls.
    • IgG anti-LPS demonstrated 88% sensitivity and 94% specificity.
    • IgA anti-LPS showed 75% sensitivity and 100% specificity at an optimal cutoff.

    Conclusions:

    • Quantitative IgA anti-LPS antibody levels are highly specific for identifying prior Salmonella LPS exposure in ReA patients.
    • These antibody measurements can aid in distinguishing ReA from other conditions in patients with a history of Salmonella infection.