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Optimized PCR-based Detection of Mycoplasma
Published on: June 20, 2011
Detection of the 10-kD antigen structural gene in mycobacteria by using the polymerase chain reaction
1Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333.
Diagnostic Microbiology and Infectious Disease
|April 1, 1994
Abstract:
The structural gene encoding the 10-kD antigen from Mycobacterium tuberculosis was amplified by the polymerase chain reaction. The 297-base-pair (bp) product was detected among 45 strains representing 14 mycobacterial species, but was absent from 11 species related to the mycobacteria. The gene was localized to a approximately 2000-bp SstII restriction fragment of the organisms' chromosomes.
