[Topical fibronectin for enhancement of burn healing]

The effects of fibronectin (Fn) on burn healing were observed in mice. A split thickness burn covered 7% approximately 8% TBSA was created on the back of mouse. Experimentally 0.05ml of Fn (0.5mg/ml) was topically applied daily. Wound healing time and healing rate were determined. On 5 and 10 postburn days, biopsy sample of wound tissue was obtained. Total protein, DNA, and hydroxyproline contents were measured according to Bradford method, Hoechest 33258 dye fluorometry, and routine measure respectively. The mean wound healing time in mice treated with Fn was 18.67 +/- 4.50 days compared to 22.89 +/- 2.85 days in the controls. The wound healing rate was significantly higher in the Fn-treated mice. The contents of wound tissue DNA and hydroxyproline in Fn group were significantly increased on day 5, and total protein was significantly increased on day 10. This study indicated that topical application of Fn is beneficial to wound healing.