Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tumor cell kinetics in mixed populations

M A Ritter1, J L Albrechtson, Y J Kim

  • 1Department of Human Oncology, University of Wisconsin Medical School, Madison.

Cytometry
|June 1, 1994
PubMed
Summary

This study developed methods to accurately measure tumor cell proliferation using flow cytometry. Techniques were validated in vitro to correct for normal cell interference, improving tumor kinetic parameter estimation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The 'Isolated' Posterior Cruciate Ligament Injury.

The Physician and sportsmedicine·2016
Same author

A Comparison of Anterior and Posterior Cruciate Ligament Laxity Between Female and Male Basketball Players.

The Physician and sportsmedicine·2016
Same author

[Not Available].

MMW Fortschritte der Medizin·2016
Same author

Micromotion at the tibial plateau in primary and revision total knee arthroplasty: fixed versus rotating platform designs.

Bone & joint research·2016
Same author

Pharmacogenetic predictor of extrapyramidal symptoms induced by antipsychotics: multilocus interaction in the mTOR pathway.

European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology·2014
Same author

Trabecular bone density of the proximal tibia as it relates to failure of a total knee replacement.

The bone & joint journal·2014

Area of Science:

  • Oncology
  • Biotechnology
  • Cell Biology

Background:

  • Accurate measurement of tumor cell proliferation is crucial for cancer treatment.
  • Normal cells in tumor biopsies can interfere with flow cytometric analysis of tumor kinetics.
  • Existing methods for estimating potential doubling times may be affected by contaminating diploid cells.

Purpose of the Study:

  • To evaluate two methods for correcting flow cytometry data affected by normal diploid cells in tumor samples.
  • To assess the reliability of the normal cell subtraction method and the percent S phase method in mixed cell populations.
  • To compare the advantages and disadvantages of these methods for in vitro and potential in vivo applications.

Main Methods:

  • Development and testing of a normal cell subtraction method using concurrent biopsies of tumor and adjacent normal tissue.

Related Experiment Videos

  • Application of flow cytometric analysis to separately process normal and tumor cell populations.
  • Evaluation of the percent S phase method, as described by Durand, in mixed and individual cell populations.
  • In vitro testing using mixtures of cell lines in various proportions.

    • Main Results:

    • Both the normal cell subtraction method and the percent S phase method provided reliable kinetic estimates in mixed in vitro cell populations.
    • The normal cell subtraction method allows for correction of normal cell interference in tumor labeling index and relative movement measurements.
    • The magnitude of interference depends on the DNA index and the proportion and labeling index of normal cells.

    Conclusions:

    • The normal cell subtraction and percent S phase methods offer viable solutions for accurate tumor cell kinetic parameter estimation.
    • These methods enhance the reliability of flow cytometry in analyzing mixed cell populations.
    • Further investigation is needed to assess the limitations of these methods for in vivo tumor measurements.