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Horseradish peroxidase: the analyst's friend
O Ryan1, M R Smyth, C O Fágáin
1School of Biological Sciences, Dublin City University, Republic of Ireland.
Essays in Biochemistry
|January 1, 1994
Summary
Horseradish peroxidase (HRP) is a stable oxidoreductase enzyme. Its diverse assay compatibility and stability make it a key indicator in various biological and chemical detection systems.
Area of Science:
- Biochemistry
- Enzymology
- Analytical Chemistry
Background:
- Horseradish peroxidase (HRP) is a 44 kDa haemoprotein.
- It is extensively glycosylated and contains calcium and four disulphide bridges.
- HRP functions as an oxidoreductase, reducing hydrogen peroxide with various hydrogen donors.
Purpose of the Study:
- To summarize the properties and applications of HRP.
- To highlight the utility of HRP as an indicator in biological assays.
Main Methods:
- Review of HRP's chemical and catalytic properties.
- Analysis of HRP's application in different assay formats.
Main Results:
- HRP exhibits stability and high catalytic rates.
- A wide range of colorimetric, fluorimetric, chemiluminescent, and electrochemical assays are available for HRP.
- HRP can be easily conjugated to other molecules.
Conclusions:
- HRP's properties facilitate its widespread use in immunoassays, DNA probes, cytochemistry, bi-enzyme systems, and biosensors.
- The enzyme's versatility and assay options contribute to its significance in diagnostics and research.