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[Age-associated changes in interleukin-1 receptors on bone marrow macrophages in ICR mouse and Wistar rat]
1Department of Pharmacology, Toho University School of Medicine, Tokyo, Japan.
Receptor levels of interleukin-1 (IL-1) on bone marrow macrophages in ICR mouse and Wistar rat were measured chronologically using europium-labeled ligand assay. The IL-1 alpha bound to high and low affinity sites but IL-1 beta bound only to low affinity site on the macrophages in both mouse and rat. The Kd values of IL-1 alpha and IL-1 beta were gradually increased with age in both species, although the binding capacities (receptor number) of IL-1 alpha were low in young and aged mice. Binding capability of IL-1 beta decreased age-dependently. These results suggested that receptors of IL-1 on bone marrow macrophages of these species might decrease with age in both quality and quantity.
Receptor levels of interleukin-1 (IL-1) on bone marrow macrophages in ICR mouse and Wistar rat were measured chronologically using europium-labeled ligand assay. The IL-1 alpha bound to high and low affinity sites but IL-1 beta bound only to low affinity site on the macrophages in both mouse and rat. The Kd values of IL-1 alpha and IL-1 beta were gradually increased with age in both species, although the binding capacities (receptor number) of IL-1 alpha were low in young and aged mice. Binding capability of IL-1 beta decreased age-dependently. These results suggested that receptors of IL-1 on bone marrow macrophages of these species might decrease with age in both quality and quantity.