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Related Experiment Videos

Jun B expression is regulated differently by three mitogenic pathways in thyrocytes

I Pirson1, J E Dumont

  • 1Institute of Interdisciplinary Research, School of Medicine, Free University of Brussels, Belgium.

Experimental Cell Research
|October 1, 1994
PubMed
Summary

Thyroid-stimulating hormone (TSH) and other growth factors regulate jun B gene expression in dog thyrocytes. TSH, via cyclic AMP, transiently increases jun B mRNA transcription, influencing cell proliferation and differentiation.

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Area of Science:

  • Molecular Endocrinology
  • Cell Biology
  • Oncogene Research

Background:

  • Thyrotropin (TSH) stimulates proliferation and differentiation in dog thyrocytes via cyclic AMP.
  • Tetradecanoylphorbol acetate (TPA) and epidermal growth factor (EGF) induce proliferation and dedifferentiation.

Purpose of the Study:

  • Investigate the regulation of protooncogene jun B mRNA expression in dog thyrocytes.
  • Determine the role of TSH, TPA, and EGF in jun B gene regulation.
  • Explore the relationship between jun B expression and cell proliferation/differentiation.

Main Methods:

  • Primary culture of dog thyrocytes
  • Nuclear run-on assays
  • mRNA accumulation studies with TSH, forskolin, TPA, EGF, cycloheximide, and actinomycin D

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Main Results:

  • TSH and forskolin induced transient, biphasic jun B mRNA expression, mediated by cyclic AMP at the transcriptional level.
  • EGF and TPA caused sustained increases in jun B mRNA levels.
  • Two jun B mRNA transcripts (2.1 and 2.3 kb) were induced by all mitogens, with distinct accumulation kinetics and potential precursor- 2.1-kb relationship.
  • jun B expression correlated with the proliferative status of thyroid cells.

Conclusions:

  • The cAMP pathway transcriptionally regulates jun B expression in dog thyrocytes.
  • Differential kinetics of jun B mRNA accumulation may relate to TSH's dual role in thyrocyte differentiation and proliferation.
  • jun B appears to be a key regulator in thyroid cell proliferation and differentiation processes.