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A method for the detection of protein-bound mutagens in food
F I Ibe1, S D Blowers, D Anderson
1BIBRA Toxicology International, Carshalton, Surrey, UK.
Food Additives and Contaminants
|May 1, 1994
Abstract:
To investigate the possible presence of protein-bound mutagens in food an analytical procedure has been devised in which the sample is enzymically hydrolysed, fractionated by HPLC and examined by a modified liquid incubation Ames assay. To validate the method MeIQx was added, as a model compound, to beefburger and a recovery of 82% obtained. The limit of detection for protein-bound mutagens was 1 microgram/kg, expressed as equivalents of MeIQx. No detectable mutagenicity was observed when the procedure was applied to samples of well cooked beefburger, irradiated chicken or mycoprotein.