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Inactivation of aminoglycosides against Pseudomonas aeruginosa by a nutrition supplementation solution
H S Ammash1, F Y Essa, V A Padron
1Department of Biology, College of Science, University of Baghdad, Jadriya, Iraq.
Abstract:
1. Possible interference of a nutritional solution (Vamin) with the activity of several aminoglycosides against Pseudomonas aeruginosa was evaluated in vitro. 2. Inactivation in cultures of 0.75, 1.2, 2.8, 65 micrograms/ml of gentamicin, kanamycin, amikacin, streptomycin, and tobramycin was induced by the addition of 1:20 v/v of the nutritional solution. 3. This inactivation was due to the presence of specific amino acids in the mixture. Deletions of amino acids from the media and amino acid analysis of the cellular pool revealed that valine, leucine, isoleucine, tyrosine, tryptophan, phenylalanine, cysteine, methionine, or threonine were responsible for the inactivation. 4. The concentration of threonine decreased in kanamycin and amikacin treated cells suggesting that certain aminoglycoside antibiotics undergo a decrease in activity when sensitive Ps. aeruginosa are treated with a nutrient solution. 5. Specific amino acids may interfere with the activity of antibiotics by circumventing their effect on amino acid biosynthesis.
Insights
Nutritional solutions can interfere with aminoglycoside antibiotics like gentamicin against Pseudomonas aeruginosa. Specific amino acids in these solutions reduce antibiotic effectiveness by impacting bacterial amino acid synthesis.
Area of Science:
- Microbiology
- Pharmacology
- Biochemistry
Background:
- Aminoglycoside antibiotics are crucial for treating Pseudomonas aeruginosa infections.
- Nutritional solutions are often administered to patients, potentially impacting drug efficacy.
Purpose of the Study:
- To investigate the in vitro interference of a nutritional solution (Vamin) with aminoglycoside activity against Pseudomonas aeruginosa.
- To identify specific components within the nutritional solution responsible for this interference.
Main Methods:
- In vitro testing of Vamin's effect on gentamicin, kanamycin, amikacin, streptomycin, and tobramycin against Pseudomonas aeruginosa cultures.
- Amino acid analysis of bacterial cellular pools and media to identify interfering substances.
Main Results:
- Vamin significantly inactivated multiple aminoglycosides against Pseudomonas aeruginosa.
- Specific amino acids including valine, leucine, isoleucine, tyrosine, tryptophan, phenylalanine, cysteine, methionine, and threonine were identified as inactivators.
- Decreased threonine concentration in treated cells suggested interference with antibiotic action.
Conclusions:
- Certain amino acids in nutritional solutions can interfere with aminoglycoside antibiotic activity against Pseudomonas aeruginosa.
- This interference may occur by circumventing the antibiotics' effects on bacterial amino acid biosynthesis.