Isolation and sequence analysis of a Caenorhabditis elegans cDNA which encodes a 14-3-3 homologue

We report the cloning of the Caenorhabditis elegans homologue of a 14-3-3 protein-encoding gene which is located within the unc-22 gene cluster on chromosome IV. Sequence analysis reveals that the cDNA-encoded product is 78% identical to both the Drosophila melanogaster and bovine 14-3-3 proteins. Our cDNA hybridizes to at least three major transcripts of 1.5, 1.35 and 0.9 kb, which are all more abundant in fertile hermaphrodites than in those lacking germ cells.