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Cloning of a somatolactin-encoding cDNA from sole (Solea senegalensis)
C Pendón1, J P Martínez-Barberá, M M Valdivia
1Department of Biochemistry and Molecular Biology, Facultad de Ciencias, Universidad de Cádiz, Spain.
Abstract:
From a Solea senegalensis cDNA expression library, clones encoding somatolactin (SL), a new pituitary hormone belonging to the growth hormone/prolactin family, were isolated and analyzed. Northern blot analysis showed a unique 1.0-kb mRNA species. The sole SL 778-bp cDNA encoded full-size S. senegalensis SL (ssSL) (230 amino acids), including seven Cys and two potential glycosylation sites. A consensus polyadenylation signal, AATAAA, was found. Protein homology and DNA sequence alignment of SL cDNAs from other evolutionarily distant marine fishes suggest that the SL sequence is highly conserved.