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Related Experiment Videos

A transducing bacteriophage lambda carrying the structural gene for elongation factor Ts

J D Friesen, J Parker, R J Watson

    Molecular & General Genetics : MGG
    |October 18, 1976
    PubMed
    Summary

    A new bacteriophage, lambda dpolC dap D-9, carries the gene for EF-Ts1. This phage enhances EF-Ts1 protein synthesis and may also contain genes for ribosomal protein S2 and the sigma factor.

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    Area of Science:

    • Molecular Biology
    • Bacteriophage Genetics
    • Protein Synthesis

    Background:

    • Bacteriophages are viruses that infect bacteria.
    • EF-Ts1 is a protein involved in protein synthesis.
    • Understanding phage-encoded genes is crucial for molecular biology.

    Purpose of the Study:

    • To isolate and characterize a specialized transducing bacteriophage.
    • To identify genes carried by the bacteriophage lambda dpolC dap D-9.
    • To investigate the phage's effect on host protein synthesis.

    Main Methods:

    • Isolation of specialized transducing bacteriophage lambda dpolC dap D-9.
    • Detection of EF-Ts1 using two-dimensional gel electrophoresis.
    • Verification of EF-Ts1 by antibody precipitation.

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  • Analysis of protein synthesis in induced lysogens.

    • Main Results:

    • The bacteriophage lambda dpolC dap D-9 carries the structural gene for EF-Ts1 (tsf).
    • EF-Ts1 was detected in proteins synthesized under phage direction in UV-inactivated cells.
    • Induced lysogens showed a 4-fold increase in EF-Ts1 synthesis.
    • Evidence suggests the phage also carries genes for ribosomal protein S2 (rpsB) and RNA polymerase sigma factor (sit).

    Conclusions:

    • The specialized transducing bacteriophage lambda dpolC dap D-9 is a valuable tool for studying EF-Ts1.
    • The phage enhances the synthesis of EF-Ts1.
    • The phage genome likely encompasses genes essential for bacterial protein synthesis and transcription machinery.