Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Morphological study on glucocorticoid-induced cataract in developing chick embryo

S Uga1, H Nishigori, S Ishikawa

  • 1Department of Ophthalmology, School of Medicine, Kitasato University, Kanagawa, Japan.

Graefe'S Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie
|July 1, 1994
PubMed
Summary

Glucocorticoid administration to chick embryos induced transient cataracts by causing oxidative stress and altering lens fiber structure. This study details the ultrastructural changes observed in the developing chick lens.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safety evaluation of the use of calcineurin inhibitor to prenatal and postpartum women in Japan from a health administrative database.

Journal of neonatal-perinatal medicine·2023
Same author

Paternal height has an impact on birth weight of their offspring in a Japanese population: the Japan Environment and Children's Study.

Journal of developmental origins of health and disease·2019
Same author

Comparison of pregnancy outcomes between women with gestational diabetes and overt diabetes first diagnosed in pregnancy: a retrospective multi-institutional study in Japan.

Diabetes research and clinical practice·2014
Same author

Identification of protein phosphatase 2C and confirmation of other protein phosphatases in the ocular lenses.

Experimental eye research·2005
Same author

Identification of protein phosphatase 2C and confirmation of other protein phosphatases in the ocular lenses.

Experimental eye research·2004
Same author

18 kDa protein tyrosine phosphatase in the ocular lens.

Experimental eye research·2001

Area of Science:

  • Ophthalmology
  • Cell Biology
  • Developmental Biology

Background:

  • Glucocorticoids are known to induce cataracts, but the underlying fine structural changes are not well-documented.
  • Previous research suggests biochemical mechanisms involving oxidative stress and protein-water phase separation.

Purpose of the Study:

  • To investigate the fine structural alterations in the chick lens following glucocorticoid administration.
  • To elucidate the cellular mechanisms contributing to glucocorticoid-induced transient cataracts.

Main Methods:

  • 15-day-old chick embryos were administered hydrocortisone succinate sodium.
  • Lens tissues were collected at various time points (12-96 hours post-administration).
  • Specimens were examined using light, transmission, and scanning electron microscopy.

Related Experiment Videos

Main Results:

  • Electron-dense cytoplasm appeared in lens fibers within 12-24 hours.
  • Vacuoles (2-8 microns) formed in intercellular spaces between 30-48 hours, correlating with opacity.
  • Epithelial cell height and fiber elongation became irregular by 96 hours.

Conclusions:

  • Transient cataracts in this model are attributed to intercellular vacuole formation.
  • Glucocorticoid exposure affects lens epithelium and equatorial lens fiber morphology.
  • The study provides crucial ultrastructural insights into glucocorticoid-induced cataractogenesis.