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Related Experiment Videos

BCG-induced mucosal immune responses

M Gheorghiu1

  • 1Laboratoire du BCG, Institut Pasteur, Paris, France.

International Journal of Immunopharmacology
|May 1, 1994
PubMed
Summary
This summary is machine-generated.

Respiratory vaccination with Bacillus Calmette-Guérin (BCG) enhances immune responses and protection against tuberculosis. Oral or respiratory administration of recombinant BCG also boosts mucosal and systemic immunity.

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Area of Science:

  • Immunology
  • Vaccinology
  • Microbiology

Background:

  • Mucosal immune responses are crucial for combating diseases like tuberculosis.
  • Bacillus Calmette-Guérin (BCG) is a primary vaccine for tuberculosis, but its administration route impacts efficacy.

Purpose of the Study:

  • To compare the immunogenicity and protective effects of BCG administered via respiratory versus intradermal routes.
  • To evaluate the immune responses elicited by recombinant BCG (LacZ-BCG) using different administration routes (oral, respiratory, intradermal).

Main Methods:

  • Guinea pigs were vaccinated with BCG via respiratory or intradermal routes.
  • Recombinant BCG expressing the LacZ gene was used to assess immune responses to a foreign antigen.
  • Immune responses including macrophage activation, lymph node proliferation, delayed type hypersensitivity, and antibody production were measured.

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  • Mice were orally immunized with recombinant BCG to detect IgA responses.

    • Main Results:

    • Aerogenic BCG vaccination led to enhanced broncho-alveolar macrophage activation and improved protection against Mycobacterium tuberculosis challenge.
    • Respiratory and oral administration of recombinant BCG induced significant mucosal and systemic immune responses, surpassing those from intradermal immunization.
    • Oral immunization with recombinant BCG in mice resulted in detectable IgA responses in both serum and intestinal secretions.

    Conclusions:

    • The respiratory route of BCG administration offers superior immune activation and protection against tuberculosis compared to the intradermal route.
    • Recombinant BCG strains administered orally or respiratorily can effectively elicit both mucosal and systemic immune responses.
    • BCG recombinants show promise as versatile platforms for developing adjuvant vaccines, enhancing immune responses through various delivery routes.