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Updated: Aug 6, 2026
A Step by Step Protocol for Subretinal Surgery in Rabbits
Published on: September 13, 2016
Vitreoretinal ablation with the 193-nm excimer laser in fluid media
D Palanker1, I Hemo, I Turovets
1Hadassah Hospital Laser Center, Hebrew University of Jerusalem, Israel.
Purpose:
To ablate retina and vitreous membranes using the 193-nm argon fluoride excimer laser in a fluid medium.
Methods:
A special delivery system for the 193-nm excimer laser was developed that enabled the delivery of the laser into high-absorption liquid environments. The system was tested on the retina in an in vitro cup preparation of cow's eyes, and also in vivo on retina and vitreous membranes of rabbit eyes. The depth of cut as a function of laser energy was determined for an ablating needle with a 0.25-mm exit diameter.
Results:
Gentle cutting of retinal tissue and of vitreous membranes was obtained in an energy range of 0.075 to 0.25 mJ/pulse. At the energy level of 0.075 mJ/pulse, four pulses were required for full-depth cut formation in rabbit retina, whereas at energy levels greater than 0.17 mJ/pulse, one pulse was sufficient for full-depth cut formation. The maximal rate of cutting achieved for the bovine retina was 2 mm/sec at a 20-Hz repetition rate of the laser. Ablation occurred only when the tip was held in contact with the tissue.
Conclusions:
The technology described herein appears to be advantageous and applicable to a variety of vitreoretinal surgical procedures.
