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T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis
Published on: July 31, 2010
Quantitative transformation of hemoglobin into stable tetramers
1Department of Biochemistry & Molecular Biophysics, College of Physicians & Surgeons of Columbia University New York, NY 10032.
Abstract:
A method is described for the preparation of human or bovine hemoglobin with a covalent bridge, formed by bispyridoxal-tetraphosphate, between the beta chains. The yield is 95% of the total hemoglobin. The location of the two molecules of bispyridoxal-tetraphosphate in the tetramer has been established. The functional properties of the cross-linked hemoglobin, its stability, and particularly, the simplicity of the method for its preparation, make it a promising candidate for an acellular blood substitute.
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