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Gastrin receptor gene expression in several human carcinomas
Y Matsushima1, Y Kinoshita, H Nakata
1Division of Gerontology, Kobe University School of Medicine.
Japanese Journal of Cancer Research : Gann
|August 1, 1994
Summary
Gastrin receptor gene expression was investigated in human carcinomas. The gastrin receptor was detected in some small cell lung, colon, and stomach cancers, suggesting its role in tumor growth.
Area of Science:
- Oncology
- Molecular Biology
- Gastroenterology
Background:
- Gastrin is known to promote the growth of several human tumors.
- Understanding gastrin's role requires investigating its receptor's presence in cancerous tissues.
Purpose of the Study:
- To examine gastrin receptor gene expression in human carcinoma cell lines.
- To analyze gastrin receptor gene expression in surgically resected carcinoma tissues.
Main Methods:
- Northern blot analysis was used to detect gastrin receptor mRNA.
- Analysis was performed on various human carcinoma cell lines and patient tissues.
Main Results:
- Gastrin receptor mRNA was detected in 3/7 small cell lung carcinoma cell lines.
- Expression was found in 1/8 colon carcinoma cell lines and 2/10 colon tissues.
- Gastrin receptor mRNA was also detected in one small cell stomach carcinoma cell line, but not in gastric adenocarcinoma tissues or cell lines.
Conclusions:
- Gastrin receptor gene expression varies across different human carcinomas.
- The findings suggest a potential role for the gastrin receptor in the growth and differentiation of specific carcinomas, including small cell lung, colon, and stomach cancers.