Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Smoothly graded Ca2+ release from inositol 1,4,5-trisphosphate-sensitive Ca2+ stores

M D Bootman1, T R Cheek, R B Moreton

  • 1Babraham Institute Laboratory of Molecular Signalling, Department of Zoology, University of Cambridge, United Kingdom.

The Journal of Biological Chemistry
|October 7, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diagnostic accuracy of proton density versus proton density fat-saturated magnetic resonance imaging (MRI) sequences in meniscal tear evaluation: a multireader ROC analysis.

Clinical radiology·2026
Same author

Replication of epidemiological associations of carpal tunnel syndrome in a UK population-based cohort of over 400,000 people.

Journal of plastic, reconstructive & aesthetic surgery : JPRAS·2021
Same author

Diagnosis and prevalence of diabetic polyneuropathy: a cross-sectional study of Danish patients with type 2 diabetes.

European journal of neurology·2020
Same author

Preoperative ultraviolet B inflammation in skin: Modelling individual differences in acute postoperative pain and neuro-immune interactions.

European journal of pain (London, England)·2017
Same author

Synchronization of activity rhythms with the tide in a saltmarsh collembolan Anurida maritima.

Oecologia·2017
Same author

Circuitry and plasticity of the dorsal horn--toward a better understanding of neuropathic pain.

Neuroscience·2015

Histamine releases intracellular calcium (Ca2+) in a dose-dependent manner, suggesting discrete calcium pools are recruited by hormones. This quantal release mechanism is not due to cell desensitization.

Area of Science:

  • Cell biology
  • Biochemistry
  • Physiology

Background:

  • Hormone stimulation causes complex intracellular calcium ion concentration ([Ca2+]i) changes.
  • Understanding calcium release from intracellular stores is crucial.

Purpose of the Study:

  • Investigate histamine's ability to release intracellular Ca2+ stores.
  • Clarify mechanisms regulating Ca2+ release under varying conditions.

Main Methods:

  • Used video imaging of single Fura-2-loaded HeLa cells.
  • Applied histamine in stepwise and pulsatile manners.
  • Conducted experiments in Ca(2+)-free and Ca(2+)-containing media at 20°C and 37°C.

Main Results:

  • Stepwise histamine increases released proportional amounts of intracellular Ca2+ in Ca(2+)-free medium, resembling quantal release.

Related Experiment Videos

  • Quantal release occurred at both temperatures and was not due to desensitization.
  • In Ca(2+)-containing medium, histamine evoked smoothly graded [Ca2+]i responses proportional to concentration.

    • Conclusions:

    • Hormone-evoked Ca2+ release from intracellular stores is limited by hormone concentration.
    • Complex [Ca2+]i signals do not result from all-or-none release of the entire Ca2+ pool.
    • The hormone-sensitive intracellular Ca2+ pool likely consists of discrete units recruited concentration-dependently.