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Related Experiment Videos

Depolymerization of hepatocellular microtubules after partial hepatectomy

V R Bommineni1, N R Chowdhury, G Y Wu

  • 1Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, New York 10461.

The Journal of Biological Chemistry
|October 7, 1994
PubMed
Summary
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Liver regeneration after partial hepatectomy disrupts microtubule networks, causing persistent endocytosis of DNA. This disruption requires protein synthesis and affects DNA clearance in hepatocytes.

Area of Science:

  • Hepatology
  • Molecular Biology
  • Cell Biology

Background:

  • Asialoglycoproteins (ASG) are internalized by hepatocytes via ASG receptor (ASGR)-mediated endocytosis.
  • Previous studies showed ASG-polylysine complexed DNA is rapidly degraded in normal rats but persists after 66% hepatectomy.

Purpose of the Study:

  • To investigate the role of hepatocellular microtubules in the persistence of endocytosed DNA following partial hepatectomy.

Main Methods:

  • Immunofluorescence confocal microscopy to examine microtubule structure.
  • Administration of cycloheximide to assess the role of protein synthesis.
  • Immunotransblot analysis to determine alpha-tubulin concentration.

Main Results:

  • Hepatocellular microtubule networks were significantly reduced 3-6 hours after 66% hepatectomy and regenerated by 24 hours.

Related Experiment Videos

  • Cycloheximide administration prevented microtubule disruption, indicating protein synthesis is required.
  • Alpha-tubulin concentration remained stable, suggesting tubulin conservation and reutilization.

    • Conclusions:

    • Partial hepatectomy-induced liver regeneration disrupts microtubule function, leading to the persistence of endocytosed DNA.
    • Protein synthesis is essential for the disassembly of microtubules during liver regeneration.
    • Microtubule integrity is crucial for the efficient degradation of internalized DNA in hepatocytes.