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Development of an ELISA assay for Clostridium perfringens phospholipase C (alpha toxin)
1Department of Surgery, Ninewells Hospital and Medical School, Dundee, United Kingdom.
Journal of Immunoassay
|August 1, 1994
Abstract:
A new method for the assay of Clostridium perfringens alpha toxin (phospholipase C) is described using a sandwich ELISA. This assay has been shown to be quantitative, to have a high specificity for the toxin and is capable of detecting purified Clostridium perfringens phospholipase C at concentrations of as little as 0.005 units/ml in cooked meat culture medium.