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Preferential decrease in thymus dependent lymphocytes during storage at 4 C in anticoagulant

J E Grunow, R A Lubet, M J Ferguson

    Transfusion
    |November 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

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    Human lymphocytes, specifically thymus-derived (T) cells, rapidly decrease in number when stored at 4°C. This study investigates the impact of storage conditions on T cell viability in blood products.

    Area of Science:

    • Immunology
    • Transfusion Medicine
    • Cell Biology

    Background:

    • Human lymphocytes are crucial for immune function.
    • Storage conditions significantly impact cell viability and function.
    • Thymus-derived (T) lymphocytes are particularly sensitive to cold storage.

    Purpose of the Study:

    • To quantify the decrease in T lymphocytes during cold storage of blood products.
    • To compare T cell loss in leukocyte concentrates (LCs) versus whole blood.
    • To investigate the influence of anticoagulant and storage duration on T cell numbers.

    Main Methods:

    • Human lymphocytes were stored at 4°C as leukocyte concentrates (LCs) or whole blood in citrate-phosphate-dextrose (CPD).
    • Cells were analyzed using Ficoll-Hypaque (F-H) density gradients.

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  • T and B lymphocyte populations were determined by cell counting and flow cytometry.

    • Main Results:

    • Stored LCs showed a rapid decline in T cells (<10% within 72 hours), with a corresponding increase in B cells.
    • LCs exposed to air showed an even faster T cell reduction.
    • Whole blood samples exhibited significant T cell loss (to 20% within 24 hours) under similar storage conditions.

    Conclusions:

    • Cold storage at 4°C leads to a substantial and rapid loss of T lymphocytes in both LCs and whole blood.
    • The observed T cell decrease is likely influenced by anticoagulant, storage duration, and blood components.
    • Findings highlight the vulnerability of T cells to standard blood storage protocols.