Current methodologies for the study of pineal morphophysiology

Light and electron microscopes, with or without the use of immunohistochemical techniques, have been the instruments of choice for study of the pineal complex even up to recent times. Other morphological technologies have become available during the past decade that, if applied to current questions concerning pineal morphophysiology, could add considerably to our understanding of this complex system. Those technologies discussed include confocal scanning laser microscopy (in conjunction with other techniques including immunohistochemistry and three-dimensional reconstruction), tissue culture methodologies, carbocyanine dyes (i.e., DiI), in situ hybridization, and application of microinjection methodologies. It is suggested that these technologies will be necessary for morphophysiologists to not only collaborate with molecular biologists and biochemists who study the pineal complex, but to corroborate the molecular and biochemical results of our colleagues.