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A revised map location for the histidine utilization genes in Pseudomonas putida
R S King1, L L Sechrist, A T Phillips
1Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802.
Journal of Basic Microbiology
|January 1, 1994
Summary
The histidine utilization (hut) genes in Pseudomonas putida were mapped to a new chromosomal location. This finding corrects previous erroneous maps and clarifies the genetic organization of the hut pathway.
Area of Science:
- Microbiology
- Bacterial Genetics
- Molecular Biology
Background:
- The histidine utilization (hut) pathway in Pseudomonas putida is essential for metabolizing histidine.
- Previous genetic mapping studies suggested the hut genes were located near 10 minutes on the chromosome.
Purpose of the Study:
- To accurately map the histidine utilization (hutH and hutU) genes in Pseudomonas putida ATCC 12633.
- To resolve discrepancies in the previously reported chromosomal location of the hut genes.
Main Methods:
- Utilized interrupted mating and transduction techniques for genetic mapping.
- Employed restriction enzyme mapping and gene cloning to confirm gene contiguity and location.
Main Results:
- The hutH and hutU genes were mapped to approximately 43 minutes on the Pseudomonas putida chromosome.
- These genes are closely linked to existing ser-800 (46 min) and met-400 (42 min) markers.
- Restriction mapping and cloning confirmed that all hut pathway genes are contiguous.
Conclusions:
- The histidine utilization genes in Pseudomonas putida are located at approximately 43 minutes, not near 10 minutes as previously reported.
- Earlier maps placing the hut genes within a superoperonic catabolic cluster at 10 minutes are incorrect.
- This study provides an accurate genetic map for the hut pathway in Pseudomonas putida.