Chloride and bicarbonate transport in chick embryonic red blood cells

1. Unidirectional efflux of 36Cl- and H14CO3- from erythrocytes of 4- to 16-day-old chick embryos was measured under steady-state conditions at 37 degrees C and pH 7.7. The efflux rates were high, > 3 s-1, and were, therefore, measured by means of the continuous flow tube method. 2. At day 4 of development the range of permeability coefficients for bicarbonate and chloride (PHCO3 and PCl was 1-30 x 10(-4) cm s-1, with average values of respectively 10 x 10(-4) and 8 x 10(-4) cm s-1. However, the results can be divided into two groups, one with PHCO3 and PCl above 12 x 10(-4) cm s-1, and one with values below 5 x 10(-4) cm s-1. The same range of values was also obtained for day 6 erythrocytes, but the overlap is more conspicuous. At day 16, PHCO3 and PCl were respectively 9 x 10(-4) and 6 x 10(-4) cm s-1 (37 degrees C, pH 7.7). In adult chicken red blood cells PHCO3 and PCl were respectively 7 x 10(-4) and 4 x 10(-4) cm s-1, and in human red blood cells the respective values were 5.6 x 10(-4) and 4 x 10(-4) cm s-1. 3. Chloride self-exchange, measured at 0 degrees C, was almost completely inhibited by addition of 1 mM 4,4'-diisothiocyanostilbene-2,2'-disulphonate (DIDS) at both days 6 and 16 of embryonic development, supporting the finding that the embryonic chick erythrocytes also have a transmembrane anion exchanger similar to that of other red cells.(ABSTRACT TRUNCATED AT 250 WORDS)