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Interaction of papillomaviruses with the cell surface
R B Roden1, R Kirnbauer, A B Jenson
1Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, Maryland 20892.
Journal of Virology
|November 1, 1994
Summary
Papillomaviruses bind to host cells via a conserved cell surface receptor, primarily involving the L1 protein. This interaction is crucial for papillomavirus infection and transformation.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Papillomaviruses cause infections and cellular changes.
- Understanding the initial steps of viral entry is key to developing interventions.
Purpose of the Study:
- To investigate the initial interaction between bovine papillomavirus type 1 (BPV) and host cells.
- To identify the viral components and cellular receptors involved in BPV binding and infection.
Main Methods:
- Binding assays using radioiodinated BPV virions and virus-like particles (VLPs).
- Focal transformation assays to assess infectivity.
- Antibody inhibition studies to determine protein involvement.
Main Results:
- BPV virions bind specifically to C127 cells in a dose-dependent manner.
- Antibodies against BPV L1 protein inhibited binding and transformation, indicating L1's role.
- Human papillomavirus type 16 (HPV-16) VLPs also inhibited BPV binding, suggesting a common receptor.
Conclusions:
- Papillomaviruses utilize a widely expressed and conserved cell surface receptor for entry.
- The L1 protein is critical for BPV binding to host cells.
- This conserved interaction suggests a common mechanism across different papillomaviruses.