[Bactericidal activities of isepamicin and ofloxacin against Pseudomonas aeruginosa evaluated using an in vitro...

Bactericidal activities of isepamicin and ofloxacin against Pseudomonas aeruginosa E7 were examined using an in vitro computer programmed pharmacokinetic simulation system. Concentration of the drugs simulated the pharmacokinetic data obtained in healthy human adult subjects after 200 mg or 400 mg I.M. administration of isepamicin (ISP), and 300 mg P.O. administration of ofloxacin (OFLX). P. aeruginosa cell counts were depressed by ISP or OFLX to -4 log -(-)5 log levels of magnitude below the initial cell counts. The time required in recovery of cell counts to the initial level after treatment with ISP (200 mg) was longer than that after OFLX (300 mg) treatment. An excellent synergistic effect was observed between ISP and OFLX.