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Related Experiment Videos

Enzyme patterns of dermatophytes

J Brasch1, M Zaldua

  • 1Department of Dermatology, University of Kiel, Germany.

Mycoses
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

Dermatophytes release various enzymes, with protein-rich media like hair stimulating the most activity. Alkaline phosphatase, esterases, and leucine arylamidase are key for fungal spread and may aid species identification.

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Area of Science:

  • Medical Mycology
  • Enzymology
  • Dermatology

Background:

  • Dermatophytes cause skin infections and fungal spread is linked to exoenzymes.
  • Understanding enzyme production is crucial for managing dermatophytosis.

Purpose of the Study:

  • To investigate enzyme release patterns by common dermatophytes and Scopulariopsis brevicaulis.
  • To correlate enzyme activity with nutrient availability and fungal growth.

Main Methods:

  • Fungi cultured in media with hair, stratum corneum, neopeptone, lipids, or RPMI.
  • Enzymes in culture supernatants analyzed using the Api-zyme test.

Main Results:

  • Protein-rich media, especially hair, stimulated the widest range of enzymes (up to 13).

Related Experiment Videos

  • Alkaline phosphatase, esterases, and leucine arylamidase were highly produced in protein media.
  • Microsporum canis showed highest total enzymatic activity; Epidermophyton floccosum showed the lowest.

    • Conclusions:

    • Alkaline phosphatase, esterases, and leucine arylamidase are likely important for dermatophyte parasitic growth.
    • Total enzymatic activity correlates with cutaneous inflammation intensity.
    • Enzyme profiles may assist in dermatophyte species identification.