Related Experiment Video
Updated: Aug 13, 2026
13:08
Expansion of Human Peripheral Blood γδ T Cells using Zoledronate
Published on: September 9, 2011
Interferon-gamma induces a decrease in the susceptibility of human glioma cells to lysis by lymphokine-activated
1Department of Neurosurgery, Utano National Hospital, Kyoto, Japan.
Neurosurgery
|July 1, 1994
Summary
Interferon-gamma treatment decreased glioblastoma cell susceptibility to lymphokine-activated killer (LAK) cells. This effect is primarily due to increased Major Histocompatibility Complex (MHC) class I antigen expression on tumor cells.
Area of Science:
- Immunology
- Oncology
- Cell Biology
Background:
- Glioblastoma is an aggressive brain tumor with limited treatment options.
- Lymphokine-activated killer (LAK) cell therapy is a potential immunotherapy for cancer.
- Understanding glioblastoma cell susceptibility to LAK cells is crucial for improving immunotherapy efficacy.
Purpose of the Study:
- To investigate the impact of interferon-gamma (IFN-gamma) on glioblastoma cell line susceptibility to LAK cell lysis.
- To examine the role of cell-adhesion molecules and MHC antigens in IFN-gamma-mediated changes in LAK cell cytotoxicity.
- To elucidate the mechanisms underlying altered glioblastoma cell recognition and killing by LAK cells.
Main Methods:
- Treatment of U-87 MG and U-251 MG glioblastoma cell lines with IFN-gamma.
- Assessment of intercellular-adhesion molecule 1 (ICAM-1), neural-cell-adhesion molecules (NCAMs), MHC class I, and MHC class II antigen expression.
- Evaluation of glioblastoma cell susceptibility to lysis by LAK cells.
- Inhibition studies using antisense-ICAM-1 oligonucleotide and acid treatment.
Main Results:
- IFN-gamma treatment increased ICAM-1 and MHC class I expression on glioblastoma cells.
- NCAMs and MHC class II expression remained high but were unaffected by IFN-gamma.
- IFN-gamma treatment decreased glioblastoma cell susceptibility to LAK cell-mediated lysis.
- Antisense-ICAM-1 further inhibited LAK lysis, while acid treatment enhanced it.
Conclusions:
- IFN-gamma treatment reduces glioblastoma cell sensitivity to LAK cell lysis.
- Increased MHC class I antigen expression is the primary mechanism for this reduced susceptibility.
- Modulating cell-adhesion molecules and MHC antigens could be a strategy to enhance LAK cell immunotherapy for glioblastoma.
Related Concept Videos
01:28Cells of the Innate Immune Response
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
01:27Cytotoxic T Cells-mediated Immune Response
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
01:29Immune Response Against Viral Pathogens
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
01:30Inhibitors of Viral Protein Synthesis
Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...