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REBASE--restriction enzymes and methylases

R J Roberts1, D Macelis

  • 1New England BioLabs, Beverly, MA 01915.

Nucleic Acids Research
|September 1, 1994
PubMed
Summary

REBASE is a comprehensive database for restriction enzymes and methylases, detailing recognition sites, cleavage sites, and commercial availability. This resource is accessible via electronic mailings, WAIS, and anonymous FTP for direct use in various software packages.

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Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Enzymology

Background:

  • Restriction enzymes and methylases are crucial tools in molecular biology.
  • Accurate and up-to-date information on these enzymes is essential for researchers.
  • Existing databases may lack comprehensive coverage or accessibility.

Purpose of the Study:

  • To present REBASE as a comprehensive database for restriction enzymes and methylases.
  • To detail the information contained within REBASE, including recognition/cleavage sites and commercial availability.
  • To outline the accessibility of REBASE data through various channels.

Main Methods:

  • Data compilation from scientific literature and commercial sources.
  • Organization of information on enzyme properties, including recognition sequences and cleavage patterns.
  • Development of accessible data distribution methods, including electronic mailings, WAIS, and anonymous FTP.

Main Results:

  • REBASE provides extensive data on restriction enzymes and methylases.
  • Information includes detailed recognition and cleavage sites for each enzyme.
  • Commercial availability of enzymes is also cataloged.
  • Specialized data files are available for direct integration with bioinformatics software.

Conclusions:

  • REBASE serves as a vital, centralized resource for researchers working with restriction enzymes and methylases.
  • The database facilitates research by providing accessible and software-compatible information.
  • Continuous updates ensure the relevance and utility of REBASE for the scientific community.

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