Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple procedure for assaying cAMP

G Munirathinam1, B C Yoburn

  • 1College of Pharmacy, St. John's University, Queens, NY 11439.

Pharmacology, Biochemistry, and Behavior
|July 1, 1994
PubMed
Summary

This study presents a cost-effective protocol for measuring cyclic adenosine monophosphate (cAMP) using a binding protein assay. The method details reagent sources, precautions, and brain tissue preparation for reliable cAMP quantification.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protective immune responses to biolistic DNA vaccination of Brugia malayi abundant larval transcript-2.

Vaccine·2012
Same author

Role of G(i)alpha2-protein in opioid tolerance and mu-opioid receptor downregulation in vivo.

Synapse (New York, N.Y.)·2002
Same author

mu-Opioid receptor downregulation contributes to opioid tolerance in vivo.

Pharmacology, biochemistry, and behavior·2001
Same author

Role of cAMP-dependent protein kinase (PKA) in opioid agonist-induced mu-opioid receptor downregulation and tolerance in mice.

Synapse (New York, N.Y.)·2000
Same author

The effect of nimodipine on opioid antagonist-induced upregulation and supersensitivity.

Pharmacology, biochemistry, and behavior·2000
Same author

In vivo regulation of mu-opioid receptor density and gene expression in CXBK and outbred Swiss Webster mice.

Synapse (New York, N.Y.)·2000

Area of Science:

  • Biochemistry
  • Neuroscience
  • Assay Development

Background:

  • Cyclic adenosine monophosphate (cAMP) is a crucial second messenger involved in numerous cellular processes.
  • Existing cAMP assays can be expensive, limiting their widespread use in research.

Purpose of the Study:

  • To develop and present a cost-effective, step-by-step protocol for quantifying cAMP.
  • To compare the efficacy of different binding proteins for cAMP detection.
  • To provide a reliable method for cAMP measurement in brain tissue.

Main Methods:

  • Adaptation of the standard commercial binding protein assay for cAMP.
  • Comparison of four distinct binding proteins for cAMP binding affinity.
  • Development of a simplified tissue preparation technique for brain samples.
  • Utilizing forskolin to stimulate cAMP production in mouse striatal tissue.

Main Results:

  • The developed protocol significantly reduces the cost per tube compared to commercial assays.
  • Identified optimal binding proteins for reliable cAMP detection.
  • Demonstrated the assay's effectiveness in measuring forskolin-induced changes in mouse brain cAMP levels.

Conclusions:

  • A validated, economical, and reliable protocol for cAMP assaying is established.
  • The method is suitable for quantifying cAMP in brain tissue, with applications in neuroscience research.
  • This assay facilitates the study of signaling pathways involving cAMP.

Related Experiment Videos