Crypt cell thymidine incorporation in ileal urinary pouch. Preliminary report

This is a brief communication about mucosal change in ileal urinary reservoirs, the earliest reported observations to date. Previous authors described early villous atrophy and crypt elongation, late panmucosal atrophy and metaplasia. Crypt cell proliferation should be a forerunner of these events and therefore warrants quantitative measurement. We studied continent pouches in dogs on postoperative days 42 and 43 using in vivo tritiated thymidine incorporation into crypt cell DNA, a novel application of autoradiography for urinary reservoirs. Adjacent normal ileum provided the internal control. Increased crypt cell thymidine incorporation was confirmed at this early time.