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Related Experiment Videos

Microtubule severing by elongation factor 1 alpha

N Shiina1, Y Gotoh, N Kubomura

  • 1Department of Genetics and Molecular Biology, Kyoto University, Japan.

Science (New York, N.Y.)
|October 14, 1994
PubMed
Summary

A novel microtubule-severing protein was identified as translational elongation factor 1 alpha (EF-1 alpha). This essential protein regulates cytoskeletal rearrangements by fragmenting microtubule arrays.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cytoskeleton Dynamics

Background:

  • Microtubule reorganization is crucial for cell cycle progression.
  • A specific protein activity responsible for severing stable microtubules is implicated in this process.

Purpose of the Study:

  • To identify the protein responsible for microtubule severing activity.
  • To investigate the role of this protein in cytoskeletal regulation.

Main Methods:

  • Purification of a 48-kilodalton microtubule-severing protein from Xenopus eggs.
  • Bacterial expression of human EF-1 alpha.
  • In vitro microtubule-severing assays.
  • Microinjection of EF-1 alpha into fibroblasts to observe effects on microtubule arrays.

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Main Results:

  • The purified microtubule-severing protein was identified as translational elongation factor 1 alpha (EF-1 alpha).
  • Bacterially expressed human EF-1 alpha demonstrated microtubule-severing activity in vitro.
  • Microinjection of EF-1 alpha into fibroblasts caused rapid and transient fragmentation of cytoplasmic microtubule arrays.

Conclusions:

  • Translational elongation factor 1 alpha (EF-1 alpha) possesses microtubule-severing activity.
  • EF-1 alpha has a dual role, acting as both a translation factor and a regulator of cytoskeletal rearrangements.
  • These findings reveal a new function for an essential component of the eukaryotic translational machinery.