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Related Experiment Videos

Phage-dependent changes in Shigella flexneri type antigen synthesis

I Financsek, I Ketyi, W Sasak

    Infection and Immunity
    |December 1, 1976
    PubMed
    Summary

    Lysogenic conversion in Shigella flexneri involves antigen changes. Wild-type phages link antigen glycosylation to glucose synthetase activity, while some mutants show a disconnect, impacting antigen formation.

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    Area of Science:

    • Microbiology
    • Molecular Biology
    • Bacteriology

    Background:

    • Shigella flexneri undergoes lysogenic conversion, a process altering its surface antigens.
    • Bacteriophages play a crucial role in mediating these genetic exchanges and phenotypic changes.

    Purpose of the Study:

    • To investigate the relationship between lysogenic conversion and antigen modification in Shigella flexneri.
    • To elucidate the role of specific phage enzymes in the synthesis of type antigens.

    Main Methods:

    • Utilized wild-type and thermosensitive mutant bacteriophages for infection experiments.
    • Assayed for polyprenyl phosphate glucose synthetase activity.
    • Monitored the appearance of glycosylated antigen and lipid-linked glucose.

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    Main Results:

    • Wild-type phages consistently showed polyprenyl phosphate glucose synthetase activity accompanying antigen glycosylation.
    • Some mutant phages exhibited glycosylated antigen without subsequent lipid-linked glucose formation.
    • Conversely, high lipid-linked glucose formation was observed with a lack of V-type antigen in some cases.

    Conclusions:

    • Lysogenic conversion significantly impacts Shigella flexneri antigenicity through phage-mediated mechanisms.
    • The polyprenyl phosphate glucose synthetase activity is closely linked to, but not solely responsible for, V-type antigen glycosylation.
    • Specific phage components are critical for the complete pathway of antigen modification during lysogenic conversion.