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Electron microscopic analysis of in vitro replication products of ors 8, a mammalian origin enriched sequence
C E Pearson1, A Shihab-el-Deen, G B Price
1McGill Cancer Centre, Department of Medicine, McGill University, Montreal, Quebec, Canada.
Abstract:
Electron microscopy was used to map the initiation site of ors 8 DNA replication in vitro in a system that is capable of initiating and supporting one round of semiconservative replication of cloned mammalian DNA origin-enriched sequences (ors). Using unique restriction sites in ors 8 plasmid DNA, we have mapped the replication bubble within the monkey DNA sequence. In addition to site-specific initiation within the ors, the results also indicate bidirectional replication.
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