Xist is expressed in female embryonal carcinoma cells with two active X chromosomes

The Xist gene resides on the X chromosome and is expressed in female but not male somatic cells. In female cells, only the Xist allele on the inactive X chromosome is transcribed. We investigated the expression of Xist in diploid P10 female embryonal carcinoma cells that have two active X chromosomes. Xist RNA was present in these P10 cells. The X chromosomes in P10 cells carry different Xist alleles whose transcripts can be distinguished by restriction digestion of their cDNAs. Both alleles were expressed. Clones of P10 cells that had lost an X chromosome did not express Xist from the remaining allele. Thus Xist is expressed in cultured cells developmentally arrested prior to X chromosome inactivation, indicating that the Xist transcript is not always derived from an inactive X chromosome. Therefore, Xist expression per se cannot be a sufficient signal to inactivate an X chromosome.