Refractive index of decapsulated bovine lens surfaces measured with a reflectometric sensor

The surface refractive index values from 16 decapsulated bovine lenses were determined with a reflectometric optical fibre sensor. The refractive index is calculated from the amount of light reflected at the interface created by the fibre and the sample. Measurements were made on anterior, posterior and equatorial surfaces. There was no difference found between values on the anterior and posterior surfaces and no variations along either surface. However index values at the equator were lower. Measurements were also conducted on sucrose solutions of graded concentrations and compared with those made using the Abbe refractometer. There was good agreement between results from the two methods.