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APL1, a yeast gene encoding a putative permease for basic amino acids

H Sychrova1, M R Chevallier

  • 1Department of Membrane Transport, Institute of Physiology, Prague, Czech Republic.

Yeast (Chichester, England)
|May 1, 1994
PubMed
Summary
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Researchers identified a new Saccharomyces cerevisiae gene, APL1 (Amino-acid Permase Like), which encodes a protein similar to known amino acid permeases. This gene is located near the LYP1 gene, suggesting a potential functional relationship in yeast.

Area of Science:

  • Molecular Biology
  • Yeast Genetics

Background:

  • The yeast Saccharomyces cerevisiae possesses various amino acid permeases for nutrient uptake.
  • Understanding the genetic regulation and function of these permeases is crucial for yeast metabolism studies.

Purpose of the Study:

  • To sequence and characterize a novel gene in Saccharomyces cerevisiae with homology to amino acid permeases.
  • To determine the location and orientation of the new gene relative to known permease genes.

Main Methods:

  • Gene sequencing of a 1722 bp DNA fragment from Saccharomyces cerevisiae.
  • Bioinformatic analysis to identify protein homology.
  • Gene mapping to determine relative positions and orientation.

Main Results:

Related Experiment Videos

  • A new gene, named APL1 (Amino-acid Permase Like), was identified, encoding a 574 amino acid protein.
  • APL1 shows high homology to basic-amino-acid permeases LYP1 and CAN1.
  • APL1 is located 881 bp upstream of the LYP1 gene in a head-to-head orientation.

    • Conclusions:

    • The discovery of APL1 expands the known family of amino acid permeases in Saccharomyces cerevisiae.
    • The proximity and orientation of APL1 to LYP1 suggest potential co-regulation or functional interaction.