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Updated: Jul 24, 2026

Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
Published on: May 23, 2011
Endogenous stimulation of lipid peroxidation in brain increases proteolytic inactivation of mitochondrial monoamine
1Institute of Biomedical Chemistry, Moscow, Russia.
Abstract:
Stimulation of lipid peroxidation in vivo (in experimental epilepsy and closed cranio-cerebral injury, as models for endogenous stimulation of lipid peroxidation) affects catalytic activity, substrate specificity of mitochondrial monoamine oxidases and increases their susceptibility to trypsinolysis. It is suggested that increased susceptibility to trypsinolysis reflects an appearance of new hydrophilic site(s) in monoamine oxidase molecules which may be responsible for an involvement of the modified enzymes in the deamination of other important nitrogenous compounds (such as gamma-aminobutyric acid) with subsequent impairment of a ratio between inhibition and excitation processes in the brain.
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