Use of lux genes in applied biochemistry

Bioluminescence has emerged in the last decade as a major tool for the study of bacterial adaptation and survival. In addition to the advantages of sensitivity and the real-time, non-invasive nature of this reporter, the imaging potential of using low-light and photon-counting video cameras has been particularly influential in establishing its ascendancy-over more traditional reporter systems. This review provides a reflection of personal activity in this field through applications in Food Microbiology and collaboration with colleagues both in the UK and beyond.