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Hydrolaparoscopy in the rabbit: a fine model for the development of operative fetoscopy
R A Quintero1, K S Puder, M Bardicef
1Department of Obstetrics and Gynecology, Hutzel Hospital, Wayne State University, Detroit, MI 48201.
Objective:
Our goal was to become adept at performing laparoscopic procedures within a fluid medium and at using miniature instruments in a small animal model.
Study Design:
Adult female New Zealand rabbits underwent carbon dioxide laparoscopy while they were under general anesthesia with the use of 2 mm instruments. The abdominal cavity was filled with lactated Ringer's solution. Visualization of the intraabdominal organs and surgical procedures were performed below the fluid level.
Results:
Excellent visualization of the abdominal organs below the fluid level was obtained. Several surgical tasks were accomplished, including cutting and coagulation of the uterine horns with monopolar electrocautery, creation of a defect in the mesovarium and mesometrium, extracorporeal knot tying, and intraabdominal cutting of suture material. Relatively high amounts of energy were needed during electrocautery within the liquid medium (> 25 W). Occasional fluid and gas leakage through the skin punctures was prevented with accessory clamps.
Conclusion:
Hydrolaparoscopy can be performed in the rabbit with miniature instruments. As it simulates the human intraamniotic environment, it is a useful model for the development of operative fetoscopy.