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Polyamines influence transglutaminase activity and cell migration in two cell lines
S A McCormack1, J Y Wang, M J Viar
1Department of Physiology and Biophysics, University of Tennessee, College of Medicine, Memphis 38163.
The American Journal of Physiology
|September 1, 1994
Summary
Polyamines are essential for cell migration, and their depletion affects transglutaminase (TGA) activity differently in IEC-6 and Caco-2 cells. Restoring polyamines normalized both migration and TGA activity in both cell lines.
Area of Science:
- Biochemistry
- Cell Biology
- Molecular Biology
Background:
- Transglutaminases (TGAs) cross-link proteins and incorporate polyamines.
- Polyamines are crucial for cell migration, as previously shown in IEC-6 cells.
- Protein-polyamine conjugates are found in various cell types.
Purpose of the Study:
- To investigate the relationship between cell migration, polyamines, and tissue TGA activity.
- To compare the effects of polyamine deficiency on TGA activity in IEC-6 and Caco-2 cells.
Main Methods:
- Utilized alpha-difluoromethylornithine (DFMO) to induce polyamine deficiency.
- Measured tissue TGA activity and protein levels via Western blot analysis.
- Assessed cell migration across a denuded surface.
Main Results:
- Both IEC-6 and Caco-2 cells require polyamines for migration.
- DFMO treatment decreased TGA activity in IEC-6 cells but increased it in Caco-2 cells.
- Polyamines restored cell migration, TGA activity, and protein levels in DFMO-treated cells.
Conclusions:
- Cell migration is dependent on polyamine levels.
- Tissue TGA activity exhibits differential responses to polyamine deficiency in different cell lines.
- Polyamines play a significant role in regulating TGA activity and cell migration.