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Related Experiment Videos

Control of intracellular pH during regulatory volume decrease in HL-60 cells

K R Hallows1, D Restrepo, P A Knauf

  • 1Department of Biophysics, University of Rochester Medical Center, New York 14642.

The American Journal of Physiology
|October 1, 1994
PubMed
Summary
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Human cells regulate intracellular pH (pHi) during volume decrease using anion exchangers and activated sodium-hydrogen exchange (NHE). This process maintains cell function under hypotonic stress.

Area of Science:

  • Cell Biology
  • Physiology
  • Biochemistry

Background:

  • Intracellular pH (pHi) homeostasis is crucial for cell function.
  • Human promyelocytic leukemic HL-60 cells exhibit regulatory volume decrease (RVD) in hypotonic conditions.
  • Understanding pHi regulation mechanisms during RVD is essential.

Purpose of the Study:

  • To investigate pHi regulation in HL-60 cells during RVD.
  • To identify the transport systems involved in maintaining pHi homeostasis.
  • To determine the role of Na+/H+ exchange (NHE) and anion exchangers (AE) in this process.

Main Methods:

  • Cell suspension in hypotonic media.
  • Measurement of intracellular pH (pHi) changes.
  • Use of transport system inhibitors (e.g., EIPA for NHE).

Related Experiment Videos

  • Assessment of ion transport rates (e.g., 22Na+ uptake).

    • Main Results:

    • Cells experienced transient intracellular acidification during early RVD.
    • Anion exchangers (AE) mediated Cl- influx and HCO3- efflux.
    • Inhibition of Na+/H+ exchange (NHE) led to significant pHi decrease.
    • NHE activity increased significantly during RVD, despite pHi and volume recovery.
    • H(+)-monocarboxylate cotransport played a minor role in pH regulation.

    Conclusions:

    • HL-60 cells effectively regulate pHi during RVD.
    • Anion exchangers (AE) and activated Na+/H+ exchange (NHE) are key players in pHi homeostasis during RVD.
    • NHE regulation is altered during hypotonic stress to facilitate pHi recovery.