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Guanylin activates rat colonic particulate guanylate cyclase
Biochemical and Biophysical Research Communications
|September 30, 1994
Summary
Guanylin activates rat colon guanylate cyclase rapidly and transiently. This activation is enhanced by adenine nucleotides like ATP, ADP, and ATPγS.
Area of Science:
- Gastroenterology
- Molecular Biology
- Cell Signaling
Background:
- Guanylin is an endogenous activator of small intestinal guanylate cyclase.
- Guanylin mRNA is abundant in rat intestinal tissues, particularly the colon.
Purpose of the Study:
- To investigate the effect of guanylin on rat colonic particulate guanylate cyclase activity.
- To determine if guanylin stimulates guanylate cyclase in intact colonocytes and isolated colonic membranes.
Main Methods:
- Incubation of intact rat colonocytes with guanylin.
- Assay of guanylate cyclase activity in isolated crude colonic membranes.
- Assessment of modulation by adenine nucleotides (ATP, ADP, ATPγS).
Main Results:
- Rat guanylin rapidly and concentration-dependently stimulated particulate guanylate cyclase in intact colonocytes.
- Guanylin also stimulated guanylate cyclase activity in isolated colonic membranes.
- ATP, ADP, and particularly ATPγS enhanced guanylin's effect on guanylate cyclase.
Conclusions:
- Guanylin is a potent, rapid stimulator of rat colonic particulate guanylate cyclase.
- Adenine nucleotides modulate the stimulatory effect of guanylin on colonic guanylate cyclase activity.