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A novel cationic amphiphile for transfection of mammalian cells
J M Ruysschaert1, A el Ouahabi, V Willeaume
1Laboratoire de Chimie Physique des Macromolécules aux Interfaces CP 206/2, Université Libre de Bruxelles, Belgium.
Abstract:
We describe here a new cationic amphiphile, N-t-butyl-N'-tetradecyl-3-tetradecylaminopropionamidine (diC14-amidine), which interacts with plasmid DNA and generates hydrophobic stable complexes resistant against DNase I. In partition experiments between two non-miscible phases, DNA was transferred into an organic phase upon complex formation with diC14-amidine-containing vesicles. Finally, vesicles made of a diC14-amidine and phosphatidylethanolamine (PE) (1:1, mol:mol) mixture or pure diC14-amidine were efficient in mediating transfection of adherent (CHO) and suspension (K562) cell lines, using the chloramphenicol acetyltransferase (CAT) gene as reporter.